Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
نویسندگان
چکیده
منابع مشابه
Tonoplast intrinsic proteins and vacuolar identity.
TIPs (tonoplast intrinsic proteins) have been traditionally used as markers for vacuolar identity in a variety of plant species and tissues. In the present article, we review recent attempts to compile a detailed map of TIP expression in Arabidopsis, in order to understand vacuolar identity and distribution in this model species. We discuss the general applicability of these findings. We also r...
متن کاملVegetative and Seed-Specific Forms of Tonoplast Intrinsic Protein in the Vacuolar Membrane of Arabidopsis thaliana.
Reports from a number of laboratories describe the presence of a family of proteins (the major intrinsic protein family) in a variety of organisms. These proteins are postulated to form channels that function in metabolite transport. In plants, this family is represented by the product of NOD26, a nodulation gene in soybean that encodes a protein of the peribacteroid membrane, and tonoplast int...
متن کاملFluorescent reporter proteins for the tonoplast and the vacuolar lumen identify a single vacuolar compartment in Arabidopsis cells.
We generated fusions between three Arabidopsis (Arabidopsis thaliana) tonoplast intrinsic proteins (TIPs; alpha-, gamma-, and delta-TIP) and yellow fluorescent protein (YFP). We also produced soluble reporters consisting of the monomeric red fluorescent protein (RFP) and either the C-terminal vacuolar sorting signal of phaseolin or the sequence-specific sorting signal of proricin. In transgenic...
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Autophagy in plant cells is induced by nutrient starvation. Initially, double membrane-bound organelles, termed autophagosomes, enclose a portion of cytoplasm, and then fuse with a vacuole or lysosome to give an autolysosome. Autolysosomes can be visualized by incubating cells in the presence of a membrane-permeable cysteine protease inhibitor. The inhibitor presumably decreases proteolytic deg...
متن کاملCharacterization in maize of ZmTIP2-3, a root-specific tonoplast intrinsic protein exhibiting aquaporin activity.
The characterization of the tonoplast intrinsic protein ZmTIP2-3 cDNA isolated from maize roots is reported. ZmTIP2-3 belongs to the TIP2 group according to the present nomenclature. The aquaporin function of ZmTIP2-3 protein was demonstrated using expression in X. laevis oocytes. Northern blot analyses revealed that ZmTIP2-3 was specifically expressed in roots. Salt and water stresses induced ...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1999
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.11.10.1867